Skip to main content
. 2004 Jan;15(1):37–45. doi: 10.1091/mbc.E03-03-0191

Figure 2.

Figure 2.

CP309 is a centrosome protein. (A) CP309, but not CG6735, is expressed in the embryos. Embryo extracts (lanes 1–4 and 7) were probed with αN1 (lane 1), αN2 (lane 2), αC1 (lane 3), αC2 (lane 4), or serum from a nonimmunized rabbit (lane 7). Sf9 cell lysates expressing FLAG-tagged CP309 protein (lane 5) and FLAG-tagged recombinant protein encoded by CG6735 (lane 6) were probed with αC2. (B) CP309 is localized to the centrosomes throughout the cell cycle. CP309 (green), microtubules (red), or γ-tubulin (red), and chromosome (blue) are shown. (C) CP309 (green), like γ-tubulin (red), localizes to the centrosomes in the absence of microtubules. Embryos were treated with colchicine before immunofluorescence staining. Bars, 5 μm.