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. 2011 Apr 12;6(4):e18547. doi: 10.1371/journal.pone.0018547

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Matsumoto et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Both strains were transformed with pMMB67HE before use. Exponentially growing cells in LB containing 100 µg/ml of AMP were harvested and resuspended in fresh LB for the assay. Nitrocefin hydrolysis was monitored by OD_{486 nm} in a 384-well plate with a plate reader after exposure to PAβN for 10 min. (A) shows results from Wild-type/pV, and (B) shows results from ΔC/pV.