Figure 2. Microscopy and mass spectrometry of Lyngbya bouillonii filaments.
(a) Light and fluorescent microscopy of a laboratory grown strain of L. bouillonii collected from Papua New Guinea. Brightfield (top) and 4′,6-diamidino-2-phenylindole (DAPI) stained and epi-fluorescent imaging (bottom) at 1000×. Cyanobacterial chlorophyll appears orange while filament sheath-associated bacterial DNA appears blue. (b) Matrix Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) of an intact L. bouillonii filament demonstrates biosynthetic production of apratoxin A (obs. [M+H]+ m/z 840).
