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(A) Reverse phase HPLC purification of synthetic feCath using a gradient of acetonitrile. Dotted line and inset indicate elution gradient. Arrow indicates positive fraction for feCath. AU-PAGE of synthetic LL-37 and feCath showing peptide homogeneity and equal peptide concentrations by Simply Blue staining (inset). (B) CD spectra of feCath (50 µM) in water (gray line) and 10 mM SDS (black line).
