Skip to main content
. 2004 Jan;15(1):71–80. doi: 10.1091/mbc.E03-08-0586

Figure 4.

Figure 4.

Identification of S. pombe Slx4 homolog. (A) Sequence alignments of Slx4 homologs from S. cerevisiae (Sc), S. pombe (Sp; SPAC688.06c), and C. albicans (C.al; Orf6.4697). Dark gray and light gray boxes indicate identical and similar residues, respectively. The peptides identified by MudPIT analysis of Slx1-TAP are underlined. (B) Fivefold serial dilutions of mutant slx1, slx4, or slx1 slx4 cells were incubated on YES agar medium supplemented with indicated amounts of MMS or HU at 30°C. To determine UV survival, cells were ascertained using a hemocytometer, plated on YES agar medium, and exposed to the indicated doses of UV. The agar plates were incubated at 30°C, and percentage of survival was calculated as the number of colonies in irradiated versus nonirradiated control. Data represent the average of two independent trials. (C). Slx4 is essential in rqh1 cells. Left, tetrad analysis of mating between slx4::kanMX6 and rqh1::ura4+ strains. An example of germination products from a slx4 rqh1 spore is presented in right panel. S, slx4; R, rqh1; RS, slx4 rqh1.