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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Mitochondrion. 2011 Feb 1;11(3):488–496. doi: 10.1016/j.mito.2011.01.005

Table 2.

tRNase ZL Processing Kinetics with Wild Type tRNAIle and Pathogenesis-Associated T Stem Mutant Substrates

tRNAIle kcata KMb kcat/KMc kcat Re WT KM Re
WT		 kcat/KM Re
WTd
WT 1.6± 0.17 1.3± 0.31 1.7± 0.5 N/A N/A N/A
m.4308G>A 0.24± 0.01 0.79± 0.05 0.31± 0.003 0.15± 0.03 0.77± 0.06 0.19± 0.02
m.4309G>A 0.48± 0.06 1.3± 0.25 0.43± 0.14 0.32± 0.04 1.21± 0.43 0.31± 0.06
a

min−1

b

X 10−8 M

c

X 108 M/min

d

Re WT (n-fold reduction re wild type) refers to the ratio of processing efficiencies: the mean kcat/KM (Mutant) / kcat/KM (WT) obtained in parallel kinetic experiments.

Averages of two or three Michaelis-Menten experiments with each variant are presented; wild type and variant experiments were performed the same day. +/− indicates standard error. Columns 1–7 are identified in the second row. Relative kcat/KM for the variants in column 7 is the n-fold reduction relative to wild type.