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. 2004 Jan;15(1):132–141. doi: 10.1091/mbc.E03-04-0215

Figure 1.

Figure 1.

Apical localization of apoB in Caco-2 cells. (A) Immunolocalization of apolipoprotein B (apoB, red channel) and sucrase-isomaltase (SI, green channel) in differentiated Caco-2 cells cultured on filters; top panels represent XY acquisitions at the apical level. Bottom panels show the corresponding XZ projections (bar, 20 μm). (B) Immunolocalization of apoB (red channel) and SI (green channel) on transverse cryosections of Caco-2 cells cultured as in A. Bar, 10 μm. (C) Immunolocalization of apoB (red channel) and phalloidin-FITC labeling of the F-actin network (green channel): top panels represent XY acquisitions at the apical level, and bottom panels show the corresponding XZ projections. Bar, 10 μm. (D) Immunolocalization of apoB (red channel) and ZO1 (green channel); top panels represent XY acquisition at the tight junctions (Tj) plane. Bottom panels show the corresponding XZ projections. Bar, 10 μm. (E) Immunoelectron microscopy analysis of apoB in the BBD of Caco-2 cells. Bar, 1 μm. (F) Schematic representation of the distribution of apoB with respect to markers.