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. Author manuscript; available in PMC: 2012 Apr 28.
Published in final edited form as: Brain Res. 2011 Mar 2;1387:141–148. doi: 10.1016/j.brainres.2011.02.078

Figure 2.

Figure 2

Oligomeric Aβ treatment down-regulates MMP2 activity in primary rat astrocytes. (A) Gelatin zymography shows MMP2 activity in astrocyte conditioned medium treated with oligomeric Aβ at indicated concentrations (hMMP2, human MMP2 standard). (B) Relative quantification of MMP2 activity in gelatin zymography (n=4 trials). (C) Real-time PCR showing MMP2 mRNA levels after oligomeric Aβ or control treatment (n=3). (D) LDH assay of the culture medium shows no significant cell toxicity induced by oligomeric Aβ in astrocyte cultures (n=3). (E) Gelatin zymography shows MMP2 activity after treatment with 20 μM oligomeric Aβ (1-42), Aβ (1-40), and reversed peptide Aβ (42-1) as compared with DMSO control (left lane).