TABLE 2. Non-labeled fatty acid compositions in rat plasma and liver (wt% of total fatty acids).
Fatty Acids | Plasma | Liver | ||||
---|---|---|---|---|---|---|
Control Diet | DHA Diet | Control Diet | DHA Diet | |||
10:0 | 0.005 ± 0.002 | 0.021 ± 0.02 | 0.009 ± 0.001 | 0.011 ± 0.001 | ||
12:0 | 0.46 ± 0.05 | 0.45 ± 0.05 | 0.34 ± 0.03 | 0.38 ± 0.04 | ||
14:0 | 1.27 ± 0.08 | 1.28 ± 0.06 | 1.27 ± 0.07 | 1.17 ± 0.06 | ||
16:0 | 17.56 ± 0.37 | 17.83 ± 0.50 | 21.25 ± 0.41 | 22.17 ± 0.59 | ||
18:0 | 12.94 ± 0.58 | 13.71 ± 0.53 | 14.86 ± 0.48 | 15.14 ± 0.53 | ||
20:0 | 0.08 ± 0.01 | 0.08 ± 0.004 | 0.04 ± 0.002 | 0.05 ± 0.02 | ||
22:0 | 0.09 ± 0.01 | 0.10 ± 0.004 | 0.05 ± 0.003 | 0.06 ± 0.003 | ||
24:0 | 0.16 ± 0.01 | 0.13 ± 0.02 | 0.16 ± 0.01 | 0.17 ± 0.01 | ||
12:1 | 0.01 ± 0.00 | ND | 0.01 ± 0.001 | 0.01 ± 0.001 | ||
14:1 | 0.09 ± 0.01 | 0.09 ± 0.004 | 0.09 ± 0.01 | 0.10 ± 0.01 | ||
16:1n-7 | 2.65 ± 0.13 | 2.27 ± 0.16 | a* | 3.07 ± 0.16 | 2.90 ± 0.19 | |
18:1n-9 | 16.16 ± 1.12 | 13.26 ± 0.95 | a** | 18.07 ± 0.78 | 16.66 ± 0.87 | a*** |
18:1n-7 | 2.51 ± 0.10 | 1.99 ± 0.09 | a*** | 3.54 ± 0.13 | 2.61 ± 0.11 | a** |
20:1n-9 | 0.13 ± 0.01 | 0.09 ± 0.005 | a*** | 0.15 ± 0.01 | 0.11 ± 0.01 | a*** |
22:1n-9 | 0.02 ± 0.00 | 0.01 ± 0.001 | a* | 0.013 ± 0.00 | 0.007 ± 0.001 | |
24:1n-9 | 0.58 ± 0.05 | 0.68 ± 0.04 | 0.10 ± 0.02 | 0.08 ± 0.005 | ||
18:3n-3 | 0.53 ± 0.03 | 0.56 ± 0.03 | 0.53 ± 0.04 | 0.61 ± 0.05 | ||
20:5n-3 | 0.57 ± 0.04 | 1.74 ± 0.11 | b*** | 0.34 ± 0.03 | 1.07 ± 0.07 | b*** |
22:5n-3 | 0.48 ± 0.03 | 0.63 ± 0.04 | b*** | 0.55 ± 0.03 | 0.71 ± 0.03 | b*** |
22:6n-3 | 4.47 ± 0.15 | 6.72 ± 0.18 | b*** | 7.52 ± 0.27 | 9.99 ± 0.33 | b*** |
24:5n-3 | ND | ND | 0.009 ± 0.001 | 0.01 ± 0.001 | b* | |
24:6n-3 | ND | ND | 0.02 ± 0.001 | 0.02 ± 0.002 | b* | |
18:2n-6 | 10.66 ± 0.41 | 12.71 ± 0.43 | b*** | 8.84 ± 0.39 | 9.89 ± 0.41 | |
18:3n-6 | 0.18 ± 0.01 | 0.17 ± 0.01 | 0.16 ± 0.01 | 0.19 ± 0.03 | ||
20:2n-6 | 0.08 ± 0.01 | 0.10 ± 0.01 | 0.11 ± 0.01 | 0.11 ± 0.01 | ||
20:3n-6 | 0.53 ± 0.03 | 0.90 ± 0.05 | b*** | 0.53 ± 0.04 | 0.77 ± 0.05 | b*** |
20:4n-6 | 23.32 ± 0.85 | 20.88 ± 0.86 | a* | 15.28 ± 0.69 | 12.32 ± 0.52 | a*** |
22:2n-6 | ND | ND | 0.02 ± 0.002 | 0.02 ± 0.001 | ||
22:4n-6 | 0.31 ± 0.02 | 0.13 ± 0.01 | a*** | 0.30 ± 0.02 | 0.15 ± 0.01 | a*** |
22:5n-6 | 0.25 ± 0.02 | 0.13 ± 0.02 | a*** | 0.31 ± 0.02 | 0.10 ± 0.01 | a*** |
24:4n-6 | ND | ND | 0.01 ± 0.001 | 0.005 ± 0.000 | a*** | |
24:5n-6 | ND | ND | 0.007 ± 0.001 | 0.002 ± 0.000 | a*** | |
Σ SFA | 32.57 ± 0.51 | 33.59 ± 0.38 | b* | 37.97 ± 0.33 | 39.16 ± 0.38 | b* |
Σ MUFA | 22.16 ± 1.12 | 18.38 ± 1.00 | a*** | 25.03 ± 0.91 | 22.47 ± 1.01 | |
Σ PUFA | 41.59 ± 0.92 | 44.95 ± 0.88 | b** | 34.48 ± 0.97 | 35.94 ± 1.10 | |
HUFA n-3 | 5.61 ± 0.16 | 9.34 ± 0.29 | b*** | 8.43 ± 0.29 | 11.81 ± 0.38 | b*** |
HUFA n-6 | 24.53 ± 0.85 | 22.08 ± 0.86 | a* | 16.46 ± 0.71 | 13.37 ± 0.54 | a*** |
HUFA n6/n3 | 4.43 ± 0.15 | 2.45 ± 0.12 | a*** | 1.95 ± 0.05 | 1.14 ± 0.03 | a*** |
Total Fatty Acids (μmol/g wet weight tissue) | ||||||
9.5 ± 0.6 | 6.9 ± 0.3 | b*** | 171.1 ± 11.4 | 160.9 ± 8.1 |
Values were presented as mean ± SEM (n=32) of endogenous fatty acids from the animals over the entire experimental period.
SFA: saturated, MUFA: monounsaturated, PUFA: polyunsaturated, HUFA: highly unsaturated fatty acids.
Significant differences were determined by two-tailed, paired t-test with P values of 0.05*, 0.01**, 0.001 or lower ***.
indicates decrement,
accretion of fatty acids in DHA diet group.