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HEV-RNA detection in PBMC by strand-specific rTth RT- PCR. (a) Negative-strand-specific assay on 10 fold serial dilutions of total RNA. Lane C, control-reaction where primer for cDNA synthesis is omitted; lane P, positive control. (b) Positive-strand-specific assay on 10 fold serial dilutions of total RNA. Lane C, control-reaction where primer for cDNA synthesis is omitted; lane P, positive control.
