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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Feb 4;1811(5):348–360. doi: 10.1016/j.bbalip.2011.01.009

Fig. 1.

Fig. 1

Coq8p is a peripheral membrane protein, associated with the mitochondrial inner membrane on the matrix side. A. Coq8p is peripherally associated with mitochondria membranes. Nycodenz purified mitochondria (M) were hypotonically swelled to generate the intermembrane space (IMS) fraction and mitoplasts. Mitoplasts were then treated with 0.1 M Na2CO3 pH 11.5, or sonicated. The treated mitoplasts were centrifuged and the supernatant (Sup) and pellet (Pel) fractions analyzed. B. Coq8p is located on the matrix side of the inner membrane. Mitochondria and mitoplasts were treated with Proteinase K with and without 1% Triton X-100. The β subunit of F1-ATPase (F1β) is a peripheral membrane protein [83], cytochrome b2 (Cyt b2) is located in the intermembrane space [84], cytochrome c1 (Cyt c1) is an integral membrane protein [85], malate dehydrogenase (Mdh1) [86], and Hsp60p [87], are localized to the matrix.