Fig. 2.
The Lysine216 in kinase subdomain II of Coq8 is required for rescue of glycerol growth in a yeast coq8 null mutant. The yeast coq8 null mutant (W303ΔABC1), was transformed with plasmids containing either wild-type COQ8 or coq8-K216A. p3HN4 (COQ8), and pK216A (coq8-K216A) are low-copy vectors; p4HN4 (COQ8), p4K216A (coq8-K216A), pBT8-1 (COQ8) and pBT8KA (coq8-K216A) are multi-copy vectors. Yeast transformants were incubated 3 days on YPG plate media at 30 °C.