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. Author manuscript; available in PMC: 2012 May 1.

Published in final edited form as: Biochim Biophys Acta. 2011 Feb 4;1811(5):348–360. doi: 10.1016/j.bbalip.2011.01.009

Fig. 5 — Amino acid alignment of Abc1/Coq8p homologues. An alignment of Abc1/Coq8p and its homologues was created using DNASTAR Megalign. Clustal method was used with PAM250 residue weight table. Homologues are E. coli (GenBank™ accession number P27854), S. pombe (GenBank™ accession number CAA62818), A. thaliana (GenBank™ accession number AAC72875), C. elegans (GenBank™ accession number AAA62560), D. melanogaster (GenBank™ accession number NP_572836) and humans (ADCK1, ADCK2, ADCK3/CABC1, ADCK4 and ADCK5. GenBank™ accession numbers NP_065154, NP_443085, BAB91363, NP_079152 and NP_777582, respectively). S. cerevisiae coq8 mutant alleles and their mutations identified in this study (Table 3) are indicated on the alignment by arrows. Mutations detected in CABC1/ADCK3 in human patients [26, 27, 73] are indicated with triangles. Kinase subdomains I, II, III, VIB, VII and VIII, the G-x-G-x-x-G glycine rich region and the D-x-x-x-x-N catalytic loop are indicated. The invariant lysine of subdomain II is designated with an asterisk. A highly conserved motif in Coq8p with remarkable sequence identity to a region in the Bacillus subtilis protein RsbU is indicated by the hatched bar (). Numbers on the left indicate amino acid position relative to the start codon. Identical residues are shaded.

Inline graphic — Amino acid alignment of Abc1/Coq8p homologues. An alignment of Abc1/Coq8p and its homologues was created using DNASTAR Megalign. Clustal method was used with PAM250 residue weight table. Homologues are E. coli (GenBank™ accession number P27854), S. pombe (GenBank™ accession number CAA62818), A. thaliana (GenBank™ accession number AAC72875), C. elegans (GenBank™ accession number AAA62560), D. melanogaster (GenBank™ accession number NP_572836) and humans (ADCK1, ADCK2, ADCK3/CABC1, ADCK4 and ADCK5. GenBank™ accession numbers NP_065154, NP_443085, BAB91363, NP_079152 and NP_777582, respectively). S. cerevisiae coq8 mutant alleles and their mutations identified in this study (Table 3) are indicated on the alignment by arrows. Mutations detected in CABC1/ADCK3 in human patients [26, 27, 73] are indicated with triangles. Kinase subdomains I, II, III, VIB, VII and VIII, the G-x-G-x-x-G glycine rich region and the D-x-x-x-x-N catalytic loop are indicated. The invariant lysine of subdomain II is designated with an asterisk. A highly conserved motif in Coq8p with remarkable sequence identity to a region in the Bacillus subtilis protein RsbU is indicated by the hatched bar (). Numbers on the left indicate amino acid position relative to the start codon. Identical residues are shaded.