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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Feb 4;1811(5):348–360. doi: 10.1016/j.bbalip.2011.01.009

Fig. 8.

Fig. 8

Expression of the human ADCK3 polypeptide in yeast coq8 mutants partially restores steady state levels of some of the yeast Coq polypeptides. Mitochondria were isolated from wild-type yeast (W303-1A) or from the indicated coq8 mutant yeast strains with or without pmcADCK3 (a multi-copy plasmid with ADCK3). Isolated mitochondria (20 μg protein) from the designated yeast strains were separated by 10% SDS-PAGE and then transferred to a PVDF membrane for immunoblotting with antibodies to one of the Coq polypeptides (Coq1-Coq9), or to malate dehydrogenase (Mdh1), as a loading control.