Table 3.
Effect of shipping back and forth at room temperature on mtDNA/nDNA quantification: correlations between mtDNA/nDNA values measured in Modena for sample sets shipped from each site back to Modena and values determined by the Modena site for their set of samples.
Site | Modena | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Samplesa (N) | 19 | 18 | 18 | 19 | 19 | 18 | 19 | 17 | 18 | 18 | 17 | 19 |
mtDNA/nDNA | ||||||||||||
Range | 25–775 | 53–966 | 95–1216 | 65–573 | 135–1689 | 157–2063 | 193–1731 | 93–743 | 53–395 | 76–699 | 68–644 | 88–2077 |
% change | N/A | +40 | +118 | +0.6 | +165 | +244 | +200 | +68 | −27 | +26 | +16 | +118 |
Pearson's b | ||||||||||||
R2 | 1.0 | 0.93 | 0.90 | 0.62 | 0.85 | 0.62 | 0.82 | 0.78 | 0.83 | 0.68 | 0.84 | 0.72 |
Slope | 1.0 | 1.07 | 1.45 | 0.42 | 1.71 | 1.56 | 1.66 | 0.83 | 0.36 | 0.55 | 0.72 | 1.69 |
p value | --- | <0.0001 | <0.0001 | <0.0001 | <0.0001 | 0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 |
In some instances, the assay did not meet the assay quality control and no value was generated.
The correlations were between the measurements made by the Modena laboratory on the set of samples assigned to their site and each of the sample sets shipped back from the participating sites.