FIGURE 1.

Basic protocol for the isolation of zebrafish skeletal muscle myogenic progenitor cells from whole dorsal myotome. Schematic showing the procedure for the isolation of skeletal myogenic progenitors from adult zebrafish dorsal muscle. Following euthanization of the zebrafish with tricaine, the fish are skinned, decapitated, de-finned, and de-gutted. A disassociation step in a mixture of collagenase IV and neutral protease breaks down cellular adhesion, whereas the use of a Ficoll gradient results in the isolation of a mononuclear cell layer. Pre-plating on uncoated plates was followed by an overnight (16-hour) transfer of the myoblast-enriched supernatant to gelatin-coated plates. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]