Table 1.
Sequences of high frequency peptides recovered from heart tissues following the third biopanning round in an ischemia-reperfusion rat model and matching motifs in candidate proteins mimicked by the phage inserts.
| Peptide Motifs (Frequency) | Matching Motifs in Candidate Protein Mimics | Accession Number (UniProtKB/Swiss-Prot) | |
|---|---|---|---|
| Ischemic Region of LV | C-STSM5LKA-C (44%) | 34834- STSKMLKA -34841 (Titin) | Q8WZ42 |
| 531- TSMLKA -536 (OPA-1) | Q2TA68 | ||
| 716-MLKA-719 (Dynamin-1 like protein, DRP-1) | O00429 | ||
| C-KPGTSSY-C (11%) | 889- CKPGTTGRYC -898 (Laminin, alpha 2) | P24043 | |
| 705- GTSSYC -710 (Brain-specific angiogenesis inhibitor 2) | O60241 | ||
| C-PDRSVNN-C (10%) | 747- DRSVENC -753 (Talin 2) | Q9Y4G6 | |
| 3869- CPDRTV -3874 (Dynein) | Q96JB1.2 | ||
| Non-ischemic Region of LV | C-WEPMNHL-C (17%) | 698- PMNHL -702 (Oxoglutarate dehydrogenase) | Q02218 |
| 961- PMTHLC -966 (Death-associated protein kinase-1) | P53355.5 | ||
| C-PFASYLH-C (16%) | 1375- PFASYL -1380 (Centlein) | Q9NXG0 | |
| 264- PFASYGLH -271 (Neutral sphingomyelin phosphodiesterase) | Q9NXE4 | ||
| C-ENVWYPR-C (11%) | 192- NIWYP -196 (Purinoceptor 4) | Q99571 | |
| 315- WFPRC (Inhibitor of apoptosis protein-1) | Q13489 | ||
| RV | C-QSTMSTL-C (15%) | 352- QSTMSTL -358 (Epidermal growth factor receptor 5) | Q86T13 |
| 132- QSTMS -136 (Tensin 4) | Q81ZW8 | ||
| C-PTSFLTD-C (10%) | 1001- CPTGFLGTDC -1010 (Multiple EGF-like domain-6) | O75095.3 | |
| 65- CPTSWVTD -72 (Cytochrome c oxidase subunit Vib polypeptide 1) | P14854.2 | ||
| C-YDLRSHQ-C (8%) | 324- CYDLRS -329 (ADAMTS-like1) | Q8N6G6 | |
| 510- YDLRSQ -515 (Schlafen family member 12) | Q8IYM2 | ||