Figure 7.

Expression of syntaxin 5 (1-43)-GFP dissociates Golgi rsly1 staining from that of 18C8. NRK cells were transfected with syntaxin 5 (1-43)-GFP, fixed, and immunostained with the indicated primary antibodies followed by cy3- and cy5-labeled secondary antibodies. Images shown used filter sets for GFP (A and G), cy3 (B, D, and H), and cy5 (C, E, and I) or a merge of cy3 and cy5 (F). D, E, and F are magnified views of the boxed region in B and C. Arrowheads mark staining in nontransfected cells, and arrows mark staining in transfected cells.