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Internalized AJ and TJ proteins transiently localize in early endosomes. Intact T84 cell monolayers or monolayers depleted of calcium for 15 and 60 min were double-immunolabeled for occludin (green) and early endosomal marker, Rab5 (red). As can be seen, internalized occludin colocalizes with the early endosomal markers at early (5 min; arrows) but not late (60 min) times of endocytosis.
