Figure 3.

Internalized AJ and TJ proteins occupy different parts of a subapical endosomal compartment. T84 cells were incubated for 60 min in S-MEM followed by double immunolabeling for ZO-1 (green) with other junctional proteins (red). In the ring-like structures, ZO-1 colocalizes (yellow) with TJ proteins occludin, JAM-1, and claudin-4 (arrows), but not with AJ proteins E-cadherin, β-catenin, and p120 catenin.