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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: J Nutr Biochem. 2010 Dec 15;22(8):777–783. doi: 10.1016/j.jnutbio.2010.07.002

Figure 1.

Figure 1

Effect of 22 and 24 substitution of the cholesterol side-chain on expression of ABCA1 and LDLR and sterol accumulation in MPMs. A) MPMs were isolated and cultured as described in Methods. On day 2, the medium was removed and replaced with medium containing carrier (Control, ethanol) or 50 μg/ml of the indicated sterols for 48h. Total cellular membranes were prepared and analyzed by immunoblotting for levels of ABCA1 and LDLR. Representative immunoblots from an experiment conducted two times are shown. B) Immunoblots were scanned and analyzed by densitometry. Sum signal intensities for ABCA1 and LDLR were normalized to Calnexin in each experiment. Bars represent the means ± standard deviations. Bars represent the means ± standard deviations. Asterisks denote significant differences from control treated cells (p < 0.01).