Figure 3.
Effect of stigmasterol on the expression of LXR (A) and SREBP1 (B), SREBP2 (C) and selected target genes in mouse peritoneal macrophages. Macrophages were elicited and cultured as in Figure 1. On day 2, the medium was removed and replaced with Medium C (Control) or Medium C supplemented with TO901317 (1 μM), sitosterol (10 μg/ml), stigmasterol (10 μg/ml), or 25-OH-C (1 μg/ml) for 24 h. Total RNA was isolated and processed for quantitative RT-PCR. Asterisks denote significant differences from control treated cells (p < 0.01).