Figure 1.

Endocytosis defects of mtm mutant strains. Shown are confocal images of wild-type, mtm-6(ar513), and mtm-9(ar479) worms either expressing pmyo-3::ssGFP (left two columns) or 2 × FYVE::GFP in their coelomocytes (right column). Worms carrying pmyo-3::ssGFP secrete GFP from their muscles into the body cavity. The coelomocytes (bright spots in the wild-type worms) endocytose the GFP from the body cavity. Defects in endocytosis result in GFP accumulating in the body cavity. The left column is a low-magnification image; the middle and last column are higher magnification images showing individual coelomocytes. Outlines of worms and coelomocytes are drawn when needed. Bar, 5 μm for the high-magnification images.