Table 3.

Spo14p-catalyzed hydrolysis of internalized BODIPY-Ptd-Cho^a

Strain	Relevant genotype	% BODIPY-PtdOH
1. Y3757	spo14Δ	0.41 ± 0.05
2. Y3248	SPO14 SEC14	5.1 ± 0.7
3. Y3247	sec14-1	2.0 ± 0.5
4. Y3756	sec14-1 spo14 Δ	0.51 ± 0.10
5. Y3528	sec14(K66A K239A)	1.5 ± 0.2
6. Y4283	sec14(K66A K239A) spo14 Δ	0.43 ± 0.08
7. Y3655	PIK1	5.0 ± 0.6
8. Y3654	pik1-11	2.0 ± 0.4
9. Y3890	pik1-11 spo14Δ	0.48 ± 0.10
10. Y3724	PIK1	5.9 ± 0.7
11. Y3723	pik1-83	1.9 ± 0.1
12. Y3907	pik1-83 spo14Δ	0.45 ± 0.08
13. Y4355	MSS4	5.3 ± 1.0
14. Y4354	mss4-2	1.9 ± 0.4
15. Y4409	mss4 spo14Δ	0.43 ± 0.10

^aThe percentage conversion of intracellular BODIPY-PtdCho to BODIPY-PtdOH was determined in cells sporulating at 34.5°C as described in MATERIALS AND METHODS. Values are means ± SD from three independent experiments