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. 2004 Jan;15(1):245–255. doi: 10.1091/mbc.E03-06-0409

Figure 2.

Figure 2.

GFP-RCC1 is highly mobile. A rectangular region of GFP-RCC1 cells was photobleached as described in the MATERIALS AND METHODS. The fluorescence inside the photobleached rectangle was then measured every 1 s for 60 s to determine the rate of recovery. (A) A comparison of the recovery rate of GFP-RCC1 fluorescence after photobleaching in interphase nuclei (top and bottom row) and a cell in metaphase (middle row). The cell in the bottom row was first energy depleted by incubation with sodium azide and 2-deoxyglucose in gluc (-) media for 20 min before observation. (B) Quantitation of the recoveries after photobleaching of GFP-RCC1 in interphase cells, metaphase cells, and energy-depleted interphase cells. Each point represents the average of 15 cells. (C) Average t1/2 (amount of time required after photobleaching to return to 50% of the final fluorescent intensity) of interphase, metaphase, and energy-depleted cells calculated from the data shown in B. The GFP-RCC1 recovery times for metaphase and energy-depleted cells were significantly different from interphase cells, p < 0.001, n = 15).