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The nucleotide requirements for GFP-RCC1 release from chromatin in permeabilized cells. Permeabilized GFP-RCC1 cells were incubated for 20 min at RT with the indicated addition before washing and fixation. Each indicated nucleotide was added at 1 mM. Cytosol (cyt) was added at 11.5 mg/ml. Detection and quantitation of GFP-RCC1 was as described in the MATERIALS AND METHODS. Between 40 and 90 nuclei were quantitated for each condition to yield the mean and SEM.
