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Dominant-negative dyn2(K44A) leads to increased Rac activity. Rat1 cells overexpressing dynamin constructs were starved in 0.4% serum for 16 h, lysed, and active Rac was assessed in a GST-PBD pull-down assay. Noninfected cells served as control. Active Rac (PBD), total Rac (WCL), and dynamin construct expression levels (HA) were detected by Western blot. Results are representative of three experiments.
