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. Author manuscript; available in PMC: 2011 Apr 13.
Published in final edited form as: Circ Cardiovasc Imaging. 2009 Nov 17;3(1):10.1161/CIRCIMAGING.109.872085. doi: 10.1161/CIRCIMAGING.109.872085

Figure 6. Ex vivo phenotyping of donor BMCs confirms inflammatory phenotype.

Figure 6

(a) Representative flow cytometry panels of saline (left) and BMC (right) injected hearts at 7 days after acute BMC delivery. At this time-point GFP+ BMCs (arrow) co-express CD45, confirming their inflammatory phenotype. (b) Serial flow cytometric analysis reveals that BMCs that were GFP+ (upper half of plot) predominantly express inflammatory cell markers (CD45, Mac-1, Gr-1), rather then stem cell (Sca-1, c-kit), endothelial progenitor cell (CD133, Flk-1) or cardiomyocyte (TropT) markers. Data are presented as percentage of GFP+ cells also positive for the markerreduced by background staining. (Error bars indicate SEM).