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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Biochemistry. 2011 Feb 9;50(10):1582–1589. doi: 10.1021/bi1018545

Table 1.

The hydride transfer barriers and enthalpy difference obtained from computer simulations of experimental T-jump kinetics.

ΔH‡L, kcal/mole ΔH‡P, kcal/mole ΔH, kcal/mole
No osmolyte 14.5±1.6 15.5±1.5 0.89±0.04
TMAO, 1 M −1.0±0.9 0.0±0.9 1.05±0.05
Urea, 1 M 9.6±2.3 9.7±2.3 0.10±0.08

ΔH‡L is the enthalpy barrier height on the ‘lactate’ side derived from the slope of k4 Eyring plot. ΔH‡P is the enthalpy barrier height on the ‘pyruvate’ side derived from k−4 Eyring plot. Barrier heights for no-osmolyte and urea systems were calculated for the temperature range 26–38 C. ΔH is the enthalpy difference derived from the Arrhenius behavior of K4 = k−4 / k4.