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Apparent barrier heights and enthalpy difference for pyruvate binding to LDH•NADH complex.
| ΔH‡OFF, kcal/mole | ΔH‡ON, kcal/mole | ΔH, kcal/mole | |
|---|---|---|---|
| No osmolyte | 14.0±0.5 | 2.0±0.4 | 12.0±0.4 |
| TMAO, 1 M | 17.3±0.5 | −3.1±0.4 | 20.3±0.5 |
| Urea, 1 M | 14.1±1.1 | 4.1±1.1 | 9.8±1.1 |
ΔH‡OFF is the enthalpy barrier derived from k6. ΔH‡ON is the enthalpy barrier derived from k−6. ΔH is the enthalpy difference derived from the Arrhenius behavior of K6 = k6 / k−6.
