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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Mol Microbiol. 2010 Oct 8;78(5):1280–1293. doi: 10.1111/j.1365-2958.2010.07407.x

Figure 1.

Figure 1

Nucleotide sequence of the nasD regulatory region. The italicized adenine residues indicate the previously identified transcription start sites (Nakano et al., 1998). The sequence of the coding strand from −114 to +2 (relative to one of the transcription start sites) is shown. Underlined are the extended −10 element, the previously identified ResD-binding (Geng et al., 2004, Nakano et al., 2000) and NsrR-binding (Nakano et al., 2006) sites. The sequence of oligonucleotides used to generate EMSA probes are also listed. The numbers in the parentheses are relative to the transcription start site. The oligonucleotide marked with ++ indicates the site where [4Fe-4S]-NsrR preferentially binds with high affinity. Apo-NsrR binds to the regions marked with +, weakly binds to the site marked with ±, does not bind to the site marked with −.