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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Mol Microbiol. 2010 Oct 8;78(5):1280–1293. doi: 10.1111/j.1365-2958.2010.07407.x

Figure 3.

Figure 3

Effect of cysteine mutations on in vivo NsrR activity.

A. Western blot analysis of the wild-type and mutant NsrR in B. subtilis. Cell lysate was prepared from a culture of an nsrR null mutant (none) as well as from the mutant carrying ectopically expressed wild-type nsrR (wt) or mutant alleles of nsrR (C92A, C100A, or C106A) at the thrC locus. The same amount of total protein from each lysate was resolved on an SDS-polyacrylamide gel and NsrR was detected by anti-NsrR antibody as described in Experimental procedures.

B. Strains carrying nasD-lacZ were grown in 2xYT supplemented with 0.5% glucose, 0.5% pyruvate, and appropriate antibiotics. Samples were collected at 1-h intervals to measure β-galactosidase activity and the activity at T1 (1 h after the end of exponential growth) is shown. The activities were measured at least three times in cultures of two independent clones and the average values of the data are presented along with standard deviations.