Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effect of spermine NONOate on DNA-binding activity of NsrR. The nasD DNA (−44 to −19) was incubated with indicated concentrations of either wild-type NsrR-His6 purified under anaerobic conditions (A), aerobic conditions (B), or C100A mutant NsrR (C). Increasing concentrations of SperNO were added to the reaction as indicated. EMSA reactions and electrophoresis were carried out in an anaerobic chamber as described in Experimental procedures.
