Figure 6.

Analysis of RNAP, ResD∼P, and NsrR binding to the nasD promoter. The nasD probe encompassing −114 and −4 of the promoter region was used. All EMSA reactions and electrophoresis were carried out in an anaerobic chamber.

A. The probe (0.2 nM) was incubated with 0.5 μM ResD phosphorylated with 0.5 μM ResE, 15 nM RNAP, or both.

B. The probe was incubated with 15 nM RNAP in the absence or presence of 0.5 μM ResD∼P and increasing concentrations of NsrR-His₆ purified under anaerobic conditions.