Fig. 2.
CD40 stimulation potentiates BCR-induced activation of PI3-K and Btk in B cells. Splenic B cells from BALB/c mice were stimulated for 5 and 15 min with F(ab’)2 anti-IgM, anti-CD40, or both, and lysed. The cell lysates were blotted with phospho-Abs against Akt, Btk, and PLCγ2, respectively. The membrane was stripped and reprobed with anti-Akt or anti-actin. One result representative of three experiments is shown.