Figure 5.

CXCL9, CXCL10 and CXCL11 but not CXCL4 stimulate migratory responses in human activated T lymphocytes. Staphylococcus enterotoxin B (SEB)/interleukin-2 (IL-2) activated T lymphocytes (3·2 × 10⁶ cells/ml) were placed on the upper membrane of a 96-well chemotaxis plate above a lower chamber containing CXCR3 agonists at the indicated concentrations as described in the Materials and methods. Cell migration across a 5-μm pore size membrane was determined after a 3 hr incubation at 37° in 5% CO₂. Cells migrating to the bottom chamber were counted using flow cytometry as described in the Materials and methods. Data shown are mean ± SEM of triplicate values from a single experiment and are representative of three other experiments using cells from different donors.