Figure 6.

Stimulation with CXCL9, CXCL10 or CXCL11, but not CXCL4, induces down-regulation of CXCR3 surface expression. Previously activated T cells (day 9–12, 1 × 10⁶/500 μl) were stimulated with increasing concentrations of CXCL4, CXCL9, CXCL10 or CXCL11 over 30 min (a), or with 100 nm of each agonist for up 1–60 min (b). Agonists were then washed off and cells were incubated with anti-CXCR3 antibody or isotype control at 4° followed by analysis FACSCanto™ flow cytometer as described in the Materials and methods. Decreases in CXCR3 surface expression is expressed as a percentage of CXCR3 levels detected on unstimulated cells. Presented data represent mean ± SEM of at least three independent experiments using cells from different donors.