Table 2.
TLR-ECD and TIR domain structuresa.
| Molecule | Residues TLR\VLR |
Resol. Å |
PDB code | Reference |
|---|---|---|---|---|
| TLR monomers | ||||
| hTLR2b | T1–284\V136–199 | 1.8 | 2Z80 | (Jin et al., 2007) |
| mTLR2/Pam3CSK4 | T27–506\V136–199 | 1.8 | 2Z81 | (Jin et al., 2007) |
| mTLR2/Pam2CSK4 | T27–506\V133–199 | 2.6 | 2Z82 | (Jin et al., 2007) |
| mTLR2/pnLTAc | T27–506\V133–200 | 2.5 | 3A7B | (Kang et al., 2009) |
| mTLR2/PE-DTPAd | T27–506\V133–200 | 2.4 | 3A7C | (Kang et al., 2009) |
| hTLR3 | T22–696 | 2.4 | 2A0Z | (Bell et al., 2005) |
| hTLR3 | T27–664 | 2.1 | 1ZIW | (Choe et al., 2005) |
| mTLR3 | T27–697 | 2.6 | 3CIG | (Liu et al., 2008) |
| hTLR4 | T27–228\V128–199 | 1.7 | 2Z62 | (Kim et al., 2007) |
| hTLR4 | T27–527\V133–199 | 2.0 | 2Z63 | (Kim et al., 2007) |
| hTLR4 | V24–82\ T228–383 | 1.9 | 2Z66 | (Kim et al., 2007) |
| hTLR4/hMD-2/Eritoran | T27–228\V19–158 | 2.7 | 2Z65 | (Kim et al., 2007) |
| mTLR4/mMD-2 | T27–625 | 2.8 | 2Z64 | (Kim et al., 2007) |
| hMD-2 | 2.0 | 2E56 | (Ohto et al., 2007) | |
| hMD-2/lipid IVa | 2.2 | 2E59 | (Ohto et al., 2007) | |
| TLR dimeric complexes | ||||
| hTLR1/hTLR2/Pam3CSK4 | T25–475\V133–199, T27–506\V133–199 | 2.1 | 2Z7X | (Jin et al., 2007) |
| mTLR3/dsRNA | T28–697 | 3.4 | 3CIY | (Liu et al., 2008) |
| hTLR4/MD-2/LPS | T27–631 | 3.1 | 3FXI | (Park et al., 2009) |
| mTLR2/mTLR6/Pam2CSK4 | T26–506\V133–200, T33–482\V157–232 | 2.9 | 3A79 | (Kang et al., 2009) |
| TIR domains | ||||
| hTIR1b | 2.9 | 1FYV | (Xu et al., 2000) | |
| hTIR2 | 3.0 | 1FYW | (Xu et al., 2000) | |
| hTIR2 P681H mutant | 2.8 | 1FYX | (Xu et al., 2000) | |
| hTIR2 C713S mutant | 3.2 | 1O77 | (Tao et al., 2002) | |
| hTIR10 | 2.2 | 2J67 | (Nyman et al., 2008) | |
| TIR (human IL-1RAPL) | 2.3 | 1T3G | (Khan et al., 2004) | |
| TIR (Arabidopsis) | 2.0 | 3JRN | (Chan et al., 2010) | |
| pdTIR (Paracoccus) | 2.5 | 3H16 | (Chan et al., 2009) | |
| TIR (MyD88) | NMR | 2Z5V | (Ohnishi et al., 2009) | |
| TIR (MyD88) | NMR | 2JS7 | unpublished | |
a
Available in the PDB as of 2010.
b
Human (h) and mouse (m) proteins with the residue numbers shown for the TLR part (T) and the VLR part (V) in case of the chimeric constructs. The length of the full TLR-ECDs including the signal peptide: TLR1 577 residues, TLR2 585, TLR3 696, TLR4 627 and TLR6 582.
c
pnLTA - S. pneumoniae lipoteichoic acid
d
PE-DTPA- 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-diethylenetriaminepentaacetic acid (synthetic phospholipid derivative)