Figure 3.
PKCα C2 domain is responsible for PKCα targeting and targets PIP2-rich regions in the plasma membrane. Images of a cell coexpressing (A) ECFP-PKCα and (B) EYFP-PKCαC2 at 15 s after treatment with 10 μM IONO are shown. (C) A merged image of A and B. Images of a cell coexpressing (D) EYFP-PKCαC2 and (E) ECFP-PLCδ1PH at 10 s after treatment with 10 μM IONO are shown. Focusing is on the dorsal surface of the cell. Insets in D and E show an enlarged area of the cell. A merged image (F) shows a smaller region of the cell. Images of a cell coexpressing (G) EYFP-PKCαC2 and (H) ECFP-PLCδ1PH at 21 s after treatment with 10 μM IONO are shown. Focusing is on the ventral surface of the cell. A merged image (I) shows overlap of images G and H. Images are representative of a minimum of five experiments.