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. 2004 Jan;15(1):371–383. doi: 10.1091/mbc.E03-05-0338

Figure 4.

Figure 4.

PKCαC2 domains containing hydrophobic residues target plasma and nuclear membranes, but not Golgi and ER. Images of a cell coexpressing (A) EYFP-PKCαC2 and (B) ECFP-PKCαC2/N189F/T250Y at 150 s after treatment with 10 μM IONO are shown. Insets in A and B show an enlarged area of the cell, including the nuclear membrane. Images of a cell coexpressing (C) EYFP-cPLA2C2 and (D) ECFP-PKCαC2/N189F/T250Y at 240 s after treatment with 10 μM IONO are shown. Arrowheads in C point to Golgi and, in D, to the same area as in C. Images are representative of at least five experiments performed on different days.