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. 2004 Jan;15(1):371–383. doi: 10.1091/mbc.E03-05-0338

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Copyright © 2004, The American Society for Cell Biology

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Figure 8. — A hybrid Syt1C2A/cPLA₂C2 domain containing cPLA₂C2 Ca²⁺-binding loops colocalizes with cPLA₂C2. (A) Ribbon diagram of the hybrid C2 domain with Ca²⁺-binding loops from cPLA₂C2 (red) on a Syt1C2A backbone (white). (B) The amino acid sequence of the hybrid shows Syt1C2A sequence (black, E140-A165, D178-T195, N203-T223, G241-K267) interrupted by insertion of cPLA₂C2 loop sequences (red, T28-P42, R61-I67, L87-I102). Images of cells coexpressing ECFP-cPLA₂C2 (C) and EYFP-Syt1C2A (D) in medium supplemented with 5 mM CaCl₂ at 55 s after treatment with 10 μM IONO are shown. (E) A merged image of C and D. Images of cells coexpressing (F) EYFP-cPLA₂C2 and (G) ECFP-Syt1C2A_cPLA₂C2_L1.2.3 in medium supplemented with 5 mM CaCl₂ at 70 s after treatment with 10 μM IONO are shown. (H) A merged image of F and G.