Table 2.
Cell type distribution of gold HDLa
| Percent distribution of gold HDL
|
||
|---|---|---|
| Cell type | Microvilli + cell surface | Caveolae + small vesicles |
| WI38[SR-BI] | 96.2 | 3.8 |
| IdlA7[SR-BI] | 96.7 | 3.3 |
| ACTH-Y1-BS1 | 99.4 | 0.6 |
| FRT/SRB1 | 93.6 | 6.4 |
a
Cells were incubated with 10 μg/ml gold HDL for 90 min on ice, washed, fixed, subjected to gold enhancement, and processed for electron microscopy as indicated in MATERIALS AND METHODS. Total number of gold particles counted were as follows: WI38[SR-BI], 2658; ldlA7[SR-BI], 729; Y1-BS1, 516; FRT/SR-BI, 1612. The distributions of gold particles between microvillar extensions and undifferentiated membrane regions for the four cell types were, respectively (in percent of total): WI38[SR-BI], 77.9%, 18.3%; ldlA7[SR-BI], 88.3%, 8.4%; ACTH-Y1-BS1, 93.6%, 5.8%; FRT/SRB1 70%, 23.6%. Micrographs were taken at 12,000× and enlarged 3× upon printing