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. 2010 Dec 1;30(48):16091–16101. doi: 10.1523/JNEUROSCI.2400-10.2010

Figure 2.

Figure 2.

Intranigral injection of poly(I:C) induces microgliosis and astrocytic activation in the dorsolateral striatum. The dorsolateral striatum was immunostained for Iba1 (green) and GFAP (green) at 4, 12, and 33 d after the intranigral injection of vehicle or 20 μg of poly(I:C). Poly(I:C) promoted microglia activation (A–D) and astrocytic reaction (E–H) within the dorsolateral striatum starting at 12 d after the injection. At day 33, activated astrocytes were still present within the dorsolateral striatum (H), whereas microglial cells showed a resting phenotype (D). Cell nuclei were counterstained with Hoechst. Scale bars: A–H, 50 μm; insets in A–D, 25 μm. I, J, Western blots of SN and striatum extracts from vehicle and poly(I:C)-injected animals. Poly(I:C) injection induced an increase of the levels of Iba1, TLR3, and CX3CR1 in the SN. Levels of Iba1 were increased in the dorsolateral striatum. Vehicle, n = 9; poly(I:C), n = 10; *p < 0.05, **p < 0.01, unpaired t test.