Figure 1. zCREST1:membRFP transgenic line allows enhanced visualization of cell shape and protrusions in FBMNs.
A: zCREST1:membRFP transgene. Expression of membrane-targeted RFP was driven by branchiomotor neuron-specific zCREST1 enhancer of the islet1 promoter. B–D Dorsal views of live zCREST1:membRFP; islet1:GFP double-transgenic 24 hpf embryos. B: zCREST1:membRFP transgene expression is limited to branchiomotor neurons, as well as a subset of cells in the otic vesicle (yellow arrowheads). C: islet1:GFP transgene. D: Merged images. D’: Higher magnification view of D, highlighting protrusions visible with membRFP expression (white arrowheads).
