Table I.
Hemodynamic and ultrasound parameters of ascending aorta and heart.
| 5–7 month-old | 23–27 month-old | |||
|---|---|---|---|---|
| Fbn1+/+ | Fbn1+/mgΔ | Fbn1+/+ | Fbn1+/mgΔ | |
| Number of Animals | 8 | 8 | 7 | 5 |
| Body Weight (g) | 29.5 ± 0.9 | 31.8 ± 0.7 * | 33.2 ±1.3 † | 31.6 ± 0.8 |
| Entire Heart weight/BW (mg/g) | 4.92 ± 0.13 | 5.99 ± 0.91 | 5.72 ± 0.35 | 6.25 ± 0.94 |
| Systolic Arterial Pressure (mmHg) | 105 ± 2 | 104 ± 3 | 110 ± 4 | 104 ± 5 |
| Mean Arterial Pressure (mmHg) | 85 ± 1 | 81 ± 2 | 84 ± 3 | 76 ± 6 |
| Diastolic Arterial Pressure (mmHg) | 75 ± 1 | 70 ± 2 | 72 ± 3 | 62 ± 6 |
| Pulse pressure (mmHg) | 30 ± 1 | 34 ± 3 | 38 ± 3 | 42 ± 2 |
| Ultrasound study | ||||
| Ascending aorta: | ||||
| Systolic Diameter (mm) | 1.70 ± 0.05 | 2.18 ± 0.17 * | 2.11 ± 0.07 † | 2.26 ± 0.19* |
| Diastolic Diameter (mm) | 1.48 ± 0.04 | 2.04 ± 0.19 * | 1.97 ± 0.08 † | 2.05 ± 0.21* |
| Surface (mm2) | 2.28 ± 0.14 | 3.90 ± 0.66 * | 3.53 ± 0.22 † | 4.09 ± 0.66 |
| Compliance (mm/mmHg) | 0.720 ± 0.137 | 0.520 ± 0.112 | 0.383 ± 0.07 ‡ | 0.579 ± 0.173 |
| Cardiac data: | ||||
| Heart Rate (bpm) | 504 ± 11 | 485 ± 25 | 475 ± 16 | 456 ± 22 |
| Left Atria Dimension (mm) | 2.7 ± 0.1 | 2.7 ± 0.1 | 2.7 ± 0.1 | 2.9 ± 0.4 |
| Left Ventricle EDD | 4.36 ± 0.18 | 5.00 ± 0.54 | 5.13 ± 0.15 † | 5.11 ± 0.26 |
| Left Ventricle EDD/BW (mm/g) | 0.148 ± 0.005 | 0.158 ± 0.017 | 0.156 ± 0.008 | 0.162 ± 0.011 |
| Fractional Shortening (%) | 40 ± 2 | 31 ± 4 | 36 ± 3 | 35 ± 4 |
| LV Weight/BW (mg/g) | 3.58 ± 0.20 | 5.40 ± 1.56 | 4.32 ± 0.24 † | 4.76 ± 0.72 |
| Vcfc (circ/s) | 2.9 ± 0.2 | 2.3 ± 0.3 | 2.5 ± 0.3 | 2.5 ± 0.2 |
| Sa (cm/s) | 2.93 ± 0.15 | 2.61 ± 0.21 | 2.84 ± 0.26 | 2.98 ± 0.50 |
| Spw (cm/s) | 2.94 ± 0.3 | 2.77 ± 0.26 | 3.22 ± 0.22 | 3.24 ± 0.22 |
| Isovolumic Relaxation Time (ms) | 17 ± 1 | 20 ± 2 | 19 ± 1 | 21 ± 1 |
| Ea (cm/s) | 4.53 ± 0.32 | 4.56 ± 0.37 | 4.74 ± 0.26 | 5.01 ± 0.77 |
| Epw (cm/s) | 4.34 ± 0.45 | 3.84 ± 0.4 | 4.97 ± 0.21 | 4.49 ± 0.49 |
| E/Ea | 20.6 ± 1.0 | 18.9 ± 2.5 | 21.6 ± 1.4 | 18.2 ± 2.4 |
| Aortic Regurgitation Frequency | 0/8 | 2/8 | 2/7 | 3/5 |
Values are mean ± sem.
†
Significant difference with 5–7 month-old animals of the same genotype (†: P≤0.05; ‡: P=0.056). Compliance is a local estimation calculated from the Δdiameter/Δpressure between systolic and diastolic values. LVEDD: left ventricle end diastolic diameter; Vcfc: mean shortening velocity of circumferential fibers, corrected for time (=FS/rate-corrected ejection time); Sa, Spw: maximal systolic velocity of the mitral annulus and posterior wall, respectively. Ea, Epw: maximal diastolic velocity of the mitral annulus and posterior wall, respectively. E/Ea: maximal velocity of LV mitral inflow to Ea.