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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Cancer Res. 2011 Jan 1;71(1):40–50. doi: 10.1158/0008-5472.CAN-10-2004

Figure 2. Increased proliferation of tumor cells in enFoxm1−/− lungs.

Figure 2

enFoxm1−/− and control Foxm1fl/fl lungs were harvested 30 weeks after initial urethane injection and used for immunohistochemistry with Ki-67 antibodies or to isolate total lung mRNA for qRT-PCR. A. Increased proliferation of tumor (Tu) cells in enFoxm1−/− lungs. B. enFoxm1−/− lungs have increased number of Ki-67-positive tumor cells compared to control mice. Ki-67-positive cells were counted in ten random microscope fields of control or enFoxm1−/− tumors. Increased mRNA levels of c-Myc (C) and Cyclin D1 (D) in enFoxm1−/− lungs after urethane treatment was demonstrated by qRT-PCR. β-actin mRNA was used for normalization. Magnifications: panels A, 100x. A p value <0.05 is shown with (*), a p value <0.01 is shown with(**).