Table 1.
Comparison of transformation frequency and integration length for purified gDNA and co-culture transformation experiments
| source of DNAa | transformation frequency (×10−6)b mean (std dev) |
integration length (bp) | |||||
|---|---|---|---|---|---|---|---|
| WT | ΔhpyR(188/CH4) | nc | WT | ΔhpyR(188/CH4) | nd | ||
| gDNA 1ng | NSH57 | 12.4 (6.19) | 22.5 (7.81) | 9 | 4295e | 5766f | 17 |
| J99 | 1.79 (0.773) | 9.10 (3.85) | |||||
| co-culture | NSH57 | 10.8 (3.17) | 4.17 (2.13) | 3 | 3592e | 5572f | 8 |
| J99 | 2.04 (0.255) | 2.51 (0.381) | |||||
a
In the co-culture experiment, ΔcomB10 mutants were used as donor strains
b
Transformation frequency calculated by dividing the number transformants by the total number of bacteria
c
number of transformation reactions
d
number of clones analyzed
e
t-test p=0.49
f
t-test p=0.87