Table 2.
Integration length (bp) in various mutants following inter-strain recombination
genotype | upstream | nc | downstream | n | overalld | n | |||
---|---|---|---|---|---|---|---|---|---|
meana | P valueb | mean | P value | mean | P value | ||||
WT | 2099 | n/ae | 27 | 1856 | n/a | 25 | 4070 | n/a | 25 |
ΔnucT | 2206 | 0.78 | 35 | 3023 | 0.03 | 35 | 5229 | 0.095 | 35 |
ΔnucT rdxA::nucT | 2135 | 0.91 | 29 | 2542 | 0.16 | 29 | 4676 | 0.38 | 29 |
ΔrecJ | 1969 | 0.68 | 47 | 2583 | 0.1 | 47 | 4503 | 0.48 | 45 |
ΔaddA | 1981 | 0.74 | 24 | 2072 | 0.68 | 25 | 4110 | 0.95 | 24 |
ΔxseA | 1631 | 0.23 | 18 | 2474 | 0.29 | 19 | 4167 | 0.9 | 18 |
ΔaddAΔrecJ | 1633 | 0.21 | 26 | 1943 | 0.87 | 27 | 3748 | 0.65 | 25 |
ΔrecG | 1298 | 0.01 | 28 | 1552 | 0.52 | 29 | 2930 | 0.06 | 27 |
ΔrecG rdxA::recG | 1585 | 0.12 | 30 | 2570 | 0.17 | 28 | 4256 | 0.79 | 28 |
ΔruvB | 1900 | 0.59 | 29 | 2342 | 0.32 | 25 | 4276 | 0.76 | 25 |
ΔruvC | 2779 | 0.07 | 29 | 2717 | 0.1 | 27 | 5598 | 0.029 | 26 |
ΔruvC rdxA::ruvC | 1954 | 0.67 | 28 | 2421 | 0.29 | 28 | 4363 | 0.68 | 27 |
ΔdprB | 2398 | 0.42 | 29 | 2311 | 0.35 | 28 | 4824 | 0.26 | 27 |
Integration length (bp) were calculated as the midpoints between informative SNPs or restriction sites. Based on the average of n clones.
t-test was used to compare the mean integration length of wild type bacteria to the mean integration length of the mutants. p-values < 0.05 are highlighted in grey.
number of clones analyzed in each recipient strain
n value for overall length is occasionally different than upstream and downstream length because clones with interspersed donor and recipient sequences that could indicate multiple recombination events were excluded.
not available