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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: J Immunol. 2010 Dec 17;186(2):791–798. doi: 10.4049/jimmunol.1002678

Figure 6. Specificity of immunosuppressive CD8+ T cells induced by AC injection of Ag depends on the Ag initially injected.

Figure 6

Wildtype B6 mice were injected AC with either 2.5 × 104 pfu HSV-1 or 5 × 105 TNP-coupled spleen cells. After 7 d, spleens were removed, CD8+ T cells were purified, and transferred to naïve B6 recipient mice (4 mice/group) that were immediately immunized with 106 pfu HSV-1 or 0.1 ml of 10 mM TNBS s.c. After another 7 d, the mice were mice were challenged with 106 UV-inactivated HSV-1 (in 33 µl PBS) or 33 µl 10 mM TNBS in the right and 33 µl PBS in the left footpad. As reference, DTH responses were measured in intact wildtype B6 mice after s.c. or AC injection of HSV-1 or TNP-spl. Values are expressed in µm (± S.E.) and represent the difference between the right (Ag challenge) and left footpad (PBS challenge). The background value represents the difference between challenged and unchallenged sites in naive mice. * p < 0.05 vs. immune control (Imm Cont.) group.