TABLE 5.
Bacterial strains and plasmids
| Strain | Genotype or descriptiona | Parent (reference) |
|---|---|---|
| LM4652 | motA2P::TnphoA opaR14 | LM5314 (Boles et al., 2000) |
| LM5093 | opaR6 | (Enos-Berlage et al., 2005) |
| LM5392 | flaM1P::EzKan opaR14 | LM5314 |
| LM5431 | opaR2 (Phosonomycinr) | (Stewart and McCarter, 2003) |
| LM5674 | ΔopaR1 | BB22OP (Enos-Berlage et al., 2005) |
| LM5738 | flgB1L::Tn5 lux opaR2 (lux aligned with transcription) | LM5431 (Stewart et al., 2003) |
| LM5839 | flhA1L::Tn5 lux opaR2 (lux not aligned) | LM5431 (Stewart and McCarter, 2003) |
| LM5841 | fliQ1L::Tn5 lux opaR2 (lux not aligned) | LM5431 (Stewart and McCarter, 2003) |
| LM6159 | vpa1598::Tn5 lux opaR2 (lux aligned) | LM5431 |
| LM6161 | vp1002::Tn5 lux opaR2 (lux aligned) | LM5431 |
| LM6209 | ΔrpoN1::Camr ΔopaR1 | LM5674 (Stewart and McCarter, 2003) |
| LM6210 | ΔfliA1L::Camr ΔopaR1 | LM5674 (Stewart et al., 2003) |
| LM6567 | Δ scrABC4::Camr | LM5674 (Ferreira et al., 2008) |
| LM7789 | ΔlafK2::Camr ΔopaR1 | LM5674 |
| LM7890 | ΔlafK3::Kanr ΔopaR1 | LM5674 |
| LM8858 | vp0575::Tn5lux opaR2 (lux aligned) | LM5431 |
| LM8906 | ΔopaR1/pLM3016 (flgC2L::lacZ) | LM5674 |
| LM8912 | flhA1L::Tn5 lux opaR2/pLM3016 (flgC2L::lacZ) | LM5839 |
| LM8913 | fliQ1L::Tn5 lux opaR2/pLM3016 (flgC2L::lacZ) | LM5841 |
| LM8933 | ΔrpoN1::Camr ΔopaR1 | LM6209 |
| LM8935 | ΔlafK3::Kanr ΔopaR1 | LM7890 |
| LM8936 | ΔopaR1/pLM3034 (lafK1::lacZ) | LM5674 |
| LM8943 | flhA1L::Tn5 lux opaR2/pLM3034 (lafK1::lacZ) | LM5839 |
| LM8944 | fliQ1L::Tn5 lux opaR2/pLM3034 (lafK1::lacZ) | LM5841 |
| LM8948 | ΔrpoN1::Camr ΔopaR1/pLM3034 (lafK1::lacZ) | LM6209 |
| LM8950 | ΔlafK3::Kanr ΔopaR1/pLM3034 (lafK1::lacZ) | LM7890 |
| LM9376 | vpa0227::Tn5 lux opaR6 (lux aligned) | LM5093 (Enos-Berlage et al., 2000) |
| LM9401 | kbl::Tn5 lux opaR6 (vpa1510=kbl gene; lux aligned) | LM5093 (Enos-Berlage et al., 2000) |
| LM9620 | vpa0227::Tn5 lux opaR6 ΔlafK2::Camr (lux aligned) | LM9376 |
| LM9889 | vpa0260::Tn5 ΔflaFAB2 ΔflaCD1708::Camr ΔopaR1 | LM4281 (Jaques, 2005) |
| LM9890 | vpa0275::Tn5 ΔflaFAB2 ΔflaCD1708::Camr ΔopaR1 | LM4281(Jaques, 2005) |
| Plasmid | ||
| pLM3016 | Tetr cosmid pLM1776 containing flgC2L::lacZ (Camr) | (Jaques, 2005) |
| pLM3034 | Tetr cosmid pLM2910 containing lafK1::lacZ (Genr) | (Stewart and McCarter, 2003) |
a
Polar (fla) and lateral (laf) flagellar genes are distinct and are indicated with the subscript P and L, respectively.